Perform a set of control reactions to determine if certain positions in the thermal cycler give low yields. Nat. Biotechnol. 16, 49–53. Varadaraj, K. and Skinner, D.M. (1994) Denaturants or cosolvents improve the specificity of PCR amplification of a G + C-rich DNA using genetically engineered DNA polymerases. These probes also can be used to detect single nucleotide polymorphisms (Lee et al. 1993; Bernard et al. 1998). There are several general categories of real-time PCR probes, including hydrolysis, hairpin and simple hybridization probes. Many Layout Posibilities 30 modules Easy to use user interface Import, export, clone templates Control header & footer contents for each tempalate Add unlimited content rows and style them individually Ability to chose a left or right sidebar for the each row in the same template. Please be aware that HTML support in mail clients varies greatly, much more so than different web browsers. We welcome contributions of HTML-ization of builtin templates.