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You can optimize your online store according to the latest requirement of search engines. The resulting solution plus ACC material then was vacuum filtered through 0.2-μm nylon membranes (Whatman) and rinsed with anhydrous ethanol (99.5%; Sigma). Filter membranes holding the captured ACC were dried subsequently overnight in a vacuum desiccator at ambient temperature. The zoomed-in window emphasizes the distinct orientation of the sensor-2 helix, the central element in linking L and S* sub-domains. ADP and the SRH* arginine finger are shown in stick representation to mark the position of the active site. Mutants predicted to decouple signaling (PS1+, PS1- and PP) were analyzed for their ATPase, unfolding, and disaggregation activities.


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